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TB-500 Calculator

A vial strength and a water volume give a concentration. A concentration and a syringe give units. Worked for the vial sizes tracked across vendors — not generic examples.

Last reviewed: September 21, 2026

Vial strength and water volume give a concentration; a dose then gives the unit mark it corresponds to.

TB-500

Vial sizes vendors carry — tap to fill:

How much bac water?
Syringe barrel

For research purposes only. Always verify calculations independently before use.

On this page

What reconstitution is

TB-500, like other research peptides, ships from vendors as a lyophilized (freeze-dried) powder sealed in a vial. Reconstitution is the act of dissolving that powder in a liquid — typically bacteriostatic water — which turns a fixed mass of peptide (the vial’s labeled mg content) into a solution with a defined concentration, measurable in volume with a syringe. The mass doesn’t change; only how that mass is distributed through a volume of liquid changes.

TB-500 is not FDA-approved by any major regulatory body, so there is no manufacturer instruction for reconstituting it. The math below is generic pharmaceutical arithmetic (concentration = mass ÷ volume), the same relationship that governs any lyophilized compound dissolved in a diluent, applied to the vial sizes TB-500 is actually sold in. For the compound’s mechanism, evidence base, and regulatory status, see the TB-500 profile.

What sizes does TB-500 come in?

Three sizes are each carried by three or more of the vendors tracked here — the sizes with a real, multi-vendor market rather than one vendor’s own catalog choice. 5 mg and 10 mg have a genuinely deep market; 2 mg clears the same three-vendor gate but only just, and is reported here at that honest depth rather than presented as equally established. Figures are read from live price data rather than a list kept by hand, which is why the calculator above offers exactly these as one-tap sizes.

Vial sizeVendors tracking this size
2 mg3
5 mg14
10 mg40

The arithmetic, worked

Concentration (mg/mL) equals the vial’s total mg divided by the water volume in mL. Micrograms per syringe unit, on a U-100 syringe (100 units = 1 mL), equals that concentration multiplied by 10. The table below reports both figures at two illustrative water volumes for each tracked size — as a statement of what each volume does to the concentration, not a recommendation of either:

Vial sizeAt 1 mL waterAt 2 mL water
2 mg2 mg/mL — 20 mcg/unit1 mg/mL — 10 mcg/unit
5 mg5 mg/mL — 50 mcg/unit2.5 mg/mL — 25 mcg/unit
10 mg10 mg/mL — 100 mcg/unit5 mg/mL — 50 mcg/unit

Doubling the water volume halves the concentration and halves the mcg delivered per unit drawn — the relationship is linear in both directions. The general dosage calculator runs this same arithmetic for any vial size and water volume, including ones outside the table above.

Units to draw, by vial size and dose

Every cell is the U-100 unit mark a given dose comes to, for a given vial strength, at 2 mL of bacteriostatic water. The rows are the vial sizes three or more tracked vendors carry; the columns are round microgram values chosen so the table reads cleanly across the range TB-500 is commonly discussed at — not a schedule. See the TB-500 profile for the evidence base and what is and isn’t established.

Vial500 mcg1000 mcg1500 mcg2000 mcg2500 mcg
2 mg50.0100.0150.0200.0250.0
5 mg20.040.060.080.0100.0
10 mg10.020.030.040.050.0

Figures over 100 are shown in amber: they exceed a 1 mL U-100 barrel at this water volume, so that combination does not fit one syringe. More water lowers the concentration and raises the unit count; less water does the reverse. The calculator above works any vial, volume and dose, in either direction.

What one vial comes to

All three tracked sizes, worked at 2 mL. “Doses per vial” is the vial’s total divided by the dose — how many times that amount is present in the vial, before any loss in handling.

10 mg vial at 2 mL — 5.00 mg/mL

DoseUnits to drawDraw volumeDoses per vial
500 mcg10.00.10 mL20.0
1000 mcg20.00.20 mL10.0
1500 mcg30.00.30 mL6.7
2000 mcg40.00.40 mL5.0
2500 mcg50.00.50 mL4.0

5 mg vial at 2 mL — 2.50 mg/mL

DoseUnits to drawDraw volumeDoses per vial
500 mcg20.00.20 mL10.0
1000 mcg40.00.40 mL5.0
1500 mcg60.00.60 mL3.3
2000 mcg80.00.80 mL2.5
2500 mcg100.01.00 mL2.0

2 mg vial at 2 mL — 1.00 mg/mL

DoseUnits to drawDraw volumeDoses per vial
500 mcg50.00.50 mL4.0
1000 mcg100.01.00 mL2.0
1500 mcg150.01.50 mL1.3
2000 mcg200.02.00 mL1.0
2500 mcg250.02.50 mL0.8

What changes the answer

  • Water volume. Concentration is inversely proportional to water volume — the table above shows this directly. A larger water volume produces a lower concentration, meaning more units are needed to reach the same mcg amount, which can exceed a syringe’s barrel capacity at low concentrations and high mcg amounts.
  • Vial size. For a fixed water volume, concentration is directly proportional to vial size — a 10 mg vial reconstituted with 1 mL is twice the concentration of a 5 mg vial reconstituted the same way.
  • Syringe calibration. Every figure above assumes a U-100 syringe (100 units = 1 mL), the standard for peptide research (see the syringes and injection guide). A different calibration — U-40, for instance — would report a different units figure for the identical physical volume; it does not change the concentration or the mcg delivered.

How long does reconstituted TB-500 last?

TB-500 is not FDA-approved by any major regulatory body and has no manufacturer label of any kind — not for the lyophilized powder, and not for a reconstituted solution.

Prof. Peptide has not identified a manufacturer label, published study, or lab document establishing how long reconstituted research-grade TB-500 remains stable — this page does not state a duration as fact. Refrigeration (2–8°C) once dissolved is standard practice, reported the same way across research peptides generally, not a figure specific to TB-500’s own stability.

FAQ

What is reconstitution?
Reconstitution is dissolving a lyophilized (freeze-dried) peptide powder in a liquid — typically bacteriostatic water — to turn it into a solution that can be measured with a syringe. The peptide's total mass doesn't change; dissolving it in liquid just makes it measurable in volume.
Does the water volume change how much peptide is in the vial?
No. A 5 mg vial contains 5 mg of TB-500 regardless of how much water is added. What changes is the concentration — milligrams per milliliter — and therefore how many syringe units correspond to a given microgram amount.
What syringe do the unit figures on this page assume?
A U-100 insulin syringe, where 100 units equals 1 mL. This is the standard syringe for peptide research — see the syringes and injection guide for barrel sizes and needle gauge.
Why doesn't this page say how much water to add or what dose to draw?
Both of those require choosing a number for the reader — a reconstitution volume and a target dose — and this page reports arithmetic, not a chosen protocol. The tables below show what a given water volume does to a given vial's concentration; how much water to use and what to inject are decisions outside what this page states.
What if my vial's size isn't in the table below?
The table covers sizes carried by 3 or more tracked vendors. A size outside that list can still be worked the same way: concentration (mg/mL) equals the vial's total mg divided by the water volume in mL, and mcg per syringe unit equals that concentration times 10 (on a U-100 syringe). The general dosage calculator performs this for any size.
How much BAC water for a 2mg TB-500 vial?
A 2 mg vial holds 2 mg of TB-500 whatever volume of bacteriostatic water is added — the water sets the concentration, not the amount in the vial. At 1 mL that vial reads 2 mg/mL, so one unit on a U-100 syringe carries 20 mcg. At 2 mL it reads 1.00 mg/mL and one unit carries 10 mcg. At 3 mL it reads 0.67 mg/mL and one unit carries 6.7 mcg. Which of those volumes to add is a choice this page does not make; the calculator above works any combination, in either direction.
How much BAC water for a 5mg TB-500 vial?
A 5 mg vial holds 5 mg of TB-500 whatever volume of bacteriostatic water is added — the water sets the concentration, not the amount in the vial. At 1 mL that vial reads 5 mg/mL, so one unit on a U-100 syringe carries 50 mcg. At 2 mL it reads 2.50 mg/mL and one unit carries 25 mcg. At 3 mL it reads 1.67 mg/mL and one unit carries 16.7 mcg. Which of those volumes to add is a choice this page does not make; the calculator above works any combination, in either direction.
How much BAC water for a 10mg TB-500 vial?
A 10 mg vial holds 10 mg of TB-500 whatever volume of bacteriostatic water is added — the water sets the concentration, not the amount in the vial. At 1 mL that vial reads 10 mg/mL, so one unit on a U-100 syringe carries 100 mcg. At 2 mL it reads 5.00 mg/mL and one unit carries 50 mcg. At 3 mL it reads 3.33 mg/mL and one unit carries 33.3 mcg. Which of those volumes to add is a choice this page does not make; the calculator above works any combination, in either direction.
How much BAC water for a 20mg TB-500 vial?
A 20 mg vial holds 20 mg of TB-500 whatever volume of bacteriostatic water is added — the water sets the concentration, not the amount in the vial. At 1 mL that vial reads 20 mg/mL, so one unit on a U-100 syringe carries 200 mcg. At 2 mL it reads 10.00 mg/mL and one unit carries 100 mcg. At 3 mL it reads 6.67 mg/mL and one unit carries 66.7 mcg. Which of those volumes to add is a choice this page does not make; the calculator above works any combination, in either direction.

References

  1. See the TB-500 profile for the compound’s evidence base, terminated Phase 2 trials, and regulatory status. This page reports arithmetic only and cites no additional source of its own beyond the generic reconstitution relationship (concentration = mass ÷ volume).

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