NAD+ Calculator

What reconstitution is

Subcutaneous, research-grade NAD+ ships from vendors as a lyophilized (freeze-dried) powder sealed in a vial — substantially larger than a typical peptide vial, at 500 mg or 1000 mg rather than a few milligrams. Reconstitution is the act of dissolving that powder in a liquid — typically bacteriostatic water — which turns a fixed mass (the vial’s labeled mg content) into a solution with a defined concentration, measurable in volume with a syringe. The mass doesn’t change; only how that mass is distributed through a volume of liquid changes.

NAD+ has no manufacturer label for research-grade material, so there is no manufacturer instruction for reconstituting it. The math below is the same generic pharmaceutical arithmetic (concentration = mass ÷ volume) used across every compound on this site, applied to the vial sizes NAD+ is actually sold in and scoped to the subcutaneous route specifically — IV, oral, and sublingual NAD+ are different formats and different dose ranges, covered on the NAD+ profile rather than here.

What sizes does NAD+ come in?

Three sizes are each carried by three or more of the vendors tracked here — the sizes with a real, multi-vendor market rather than one vendor’s own catalog choice. 500 mg and 1000 mg have a genuinely deep market; 100 mg clears the same three-vendor gate but only just, and is reported here at that honest depth. Figures are read from live price data rather than a list kept by hand, which is why the calculator above offers exactly these as one-tap sizes.

The arithmetic, worked

Concentration (mg/mL) equals the vial’s total mg divided by the water volume in mL. Milligrams per syringe unit, on a U-100 syringe (100 units = 1 mL), equals that concentration divided by 100. The table below reports both figures at two illustrative water volumes for each tracked size — larger volumes than the peptide pages on this site, since NAD+ vials and doses are both substantially larger — as a statement of what each volume does to the concentration, not a recommendation of either:

Increasing the water volume lowers the concentration and lowers the mg delivered per unit drawn — the relationship is linear in both directions. The general dosage calculator runs this same arithmetic for any vial size and water volume, including ones outside the table above.

Units to draw, by vial size and dose

Every cell is the U-100 unit mark a given dose comes to, for a given vial strength, at 2 mL of bacteriostatic water — the profile’s own stated reconstitution convention for NAD+. The rows are the vial sizes three or more tracked vendors carry; the columns are round milligram values spanning the profile’s subcutaneous range specifically (50–100 mg), not its IV, oral, or sublingual figures, which are different routes. Not a schedule. See the NAD+ profile for the full set of routes and figures.

Figures over 100 are shown in amber: they exceed a 1 mL U-100 barrel at this water volume, so that combination does not fit one syringe — this only happens on the thin 100 mg vial at the higher doses in this table. More water lowers the concentration and raises the unit count; less water does the reverse. The calculator above works any vial, volume and dose, in either direction.

What one vial comes to

All three tracked sizes, worked at 2 mL. “Doses per vial” is the vial’s total divided by the dose — how many times that amount is present in the vial, before any loss in handling.

What changes the answer

• Water volume. Concentration is inversely proportional to water volume — the table above shows this directly. A larger water volume produces a lower concentration, meaning more units are needed to reach the same mg amount, which can exceed a syringe’s barrel capacity at low concentrations and high mg amounts — the reason NAD+’s own reconstitution convention favors 1–2 mL rather than a larger volume.
• Vial size. For a fixed water volume, concentration is directly proportional to vial size — a 1000 mg vial reconstituted with 2 mL is twice the concentration of a 500 mg vial reconstituted the same way.
• Syringe calibration. Every figure above assumes a U-100 syringe (100 units = 1 mL), the standard for peptide and NAD+ research (see the syringes and injection guide). A different calibration would report a different units figure for the identical physical volume; it does not change the concentration or the mg delivered.

How long does reconstituted NAD+ last?

FAQ

References

1. See the NAD+ profile for the evidence base across all administration routes. This page reports arithmetic only, scoped to the subcutaneous route, and cites no additional source of its own beyond the generic reconstitution relationship (concentration = mass ÷ volume).